Fig. 6
From: STK16 promoted colorectal cancer progress in a c-MYC signaling-dependent manner
c-MYC S452 Phosphorylation Was Essential for STK16-Mediated Cancer Cell Proliferation. A. IB assays detected the expression level of identified proteins in colorectal cancer cells stably expressing vector + shnc, STK16 + shnc, STK16 + shc-MYC#1, or STK16 + shc-MYC#2. B-D. CCK8 (B) and Brdu assays (C, D) assessed the proliferation ability of identified cancer cells. E. IB assays detected the expression level of identified proteins in colorectal cancer cells stably expressing vector + c-MYC WT, STK16 + c-MYC WT, vector + c-MYC S452A, or STK16 + c-MYC S452A. F-H. CCK8 (F) and Brdu assays (G, H) assessed the proliferation ability of identified cancer cells. All IB assays were conducted three times, and consistent results were obtained. Statistical analysis used Student’s t-test