Fig. 1From: Bleomycin induces senescence and repression of DNA repair via downregulation of Rad51Bleomycin causes cellular senescence in AECs. A, B A549 and MLE-12 cells were treated with different concentrations of bleomycin for 3 days. SAâβâgal staining was performed to detect cellular senescence (magnificationâĂ100). C, D The expression of senescence-related markers p21WAF1 and p16ink4a in A549 and MLE-12 cells was analyzed by Western blotting. E, F The relative mRNA expression of SASP factors (IL-1Îą, IL-1β, IL-8, and CXCL-1) in A549 and MLE-12 cells was analyzed by RT-PCR. G EdU incorporation assay was performed to detect A549 cell proliferation and the EdU-positive cells were quantitated. Significance markers: *pâ<â0.05; **pâ<â0.01; ***pâ<â0.001 compared to control; nâ=â3Back to article page