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Fig. 1 | Molecular Medicine

Fig. 1

From: An Inhibitor of Macrophage Arginine Transport and Nitric Oxide Production (CNI-1493) Prevents Acute Inflammation and Endotoxin Lethality

Fig. 1

Dependence of RAW cells on extracellular arginine to support NO productiona

RAW 264.7 cells were plated in RPMI devoid of L-arginine or in RPMI supplemented with L-arginine as indicated, then stimulated by the addition of LPS (100 ng/ml) and IFNγ (25 U/ml) at time zero. Total nitrite accumulated in the medium conditioned by 1 × 106 cells over the subsequent 18 hr is shown. Assays were performed in triplicate, and the experiment repeated at least three times. Data shown are mean ± SEM (n = 3 experiments).

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