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Fig. 3 | Molecular Medicine

Fig. 3

From: Nramp Transfection Transfers Ity/Lsh/Bcg-Related Pleiotropic Effects on Macrophage Activation: Influence on Oxidative Burst and Nitric Oxide Pathways

Fig. 3

Resistant allele RAW 264.7 transfectants exhibit enhanced NO generation in response to LPS (a) or IFN γ + LPS (b)

Cells were incubated for 24 or 30 hr in the presence of LPS (25 ng/ml) or LPS plus IFNγ (25 ng/ml and 25 U/ml). Determinations were normalized to cell number from the crystal violet staining intensity of a parallel plate, and results are presented as the ratio of nitrite to crystal violet. Asterisks indicate significance levels (*p < 0.05; **p < 0.01; ***p < 0.001) for results of Student’s t tests used to compare each resistant transfectant against the susceptible transfectants 10S and 30S. Clones 17.3R and 17.6R also showed significantly higher (p < 0.05) nitrite levels in this experiment. Results are representative of five independent experiments performed.

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