Fig. 2

Transactivation activities of WT and mutant h-TR β 1, ED, OK, and PV on Lys-TRE in HeLa cells

HeLa cells were transfected with the respective h-TRβ1 and 5 µg of TK-TRE-CAT plasmids and the cells treated with the appropriate T₃ concentrations 24 hr later. CAT activities were normalized to that obtained by WT h-TRβ1 at 1000 nM T₃. Results of two experiments, each with duplicate plates per receptor/T₃ concentration were expressed as relative CAT activity (%) ± SEM.