Gel mobility analyses using nuclear extracts from HeLa cells and NIH3T3 cells with mutant receptor OK on the (A) Lys- and (B) ME-TREs
Experimental conditions were identical to those for Fig. 6. In this case, however, both gels were exposed for 14 hr. There is a nonspecific band (NS) that runs just a little above the homodimer band in the panel with HeLa nuclear extract and again, as in Fig. 6A, is not competed away by specific competitor. (▷) The lower band in Lane 5 likely represents a faster migrating heterodimer band competed away by specific competitor (Lane 6) and overlaps a nonspecific band. As in Fig. 6, the Panels A and B each represent a gel, respectively.