| |
Monkeysb
|
Prepatent Periodc (days)
|
Coursed
|
Invasione (% Infected RBCs)
|
IIFf
|
ELISAg
|
|---|
|
Vaccinea
|
#
|
Sex
|
Species
|
|---|
|
Freund’s alone
|
701
|
Male
|
N
|
9
|
Virulent
|
1.5
|
<102
|
<102
|
|
886
|
Female
|
N
|
12
|
Virulent
|
1.7
|
<102
|
<102
|
|
184
|
Male
|
V
|
7
|
Virulent
|
1.3
|
<102
|
<102
|
|
yMSP119
|
331A
|
Male
|
N
|
11
|
Self-resolved
|
1.6
|
104
|
106
|
|
218
|
Female
|
N
|
12
|
Self-resolved
|
1.6
|
104
|
105
|
|
1193
|
Male
|
V
|
28
|
Virulent
|
1.7
|
>105
|
106
|
|
2458
|
Male
|
V
|
11
|
Virulent
|
2.1
|
104
|
106
|
|
bGST-MSP142
|
202
|
Male
|
N
|
10
|
Virulent
|
ND
|
103
|
103
|
|
E851
|
Male
|
N
|
11
|
Persistent
|
ND
|
103
|
104
|
|
T212
|
Male
|
V
|
8
|
Virulent
|
ND
|
104
|
104
|
- ayMSP119, yeast produced fusion of the 19-kD C-terminus of MSP119; GST-MSP142, bacterial produced 42-kD C-terminus of MSP1 fused with glutathione-S-transferase.
- bSpecies of Aotus: N, A. nancymai; V, A. vociferans.
- c Prepatent period: time from inoculation of parasites to first visualized parasite in the blood.
- dVirulent, rose to above 5%, requiring treatment; self-resolved, after a low-grade parasitemia of <0.1%; Persistent, no control, but parasitemia always below 5%.
- ePercentage of red cells that contained ring-stage parasites in the invasion assay performed in the presence of Aotus sera from Day 17 after the third immunization (the day of parasite challenge).
- fIIF, indirect immunofluorescence against FCRIII that has the identical C-terminus as the FVO strain of P. falciparum.
- gELISA, enzyme-linked immunosorbent assay. End-point titers defined as the highest dilution of sera with an A405 of 0.4 or greater (data from three different assays).
