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Table 1 The Relation Between the Course of Infection in Vaccinated Aotus Monkeys and Neutralization of Invasion and Other Serologic Studies

From: Immunogenicity and In Vivo Efficacy of Recombinant Plasmodium falciparum Merozoite Surface Protein-1 in Aotus Monkeys

  Monkeysb Prepatent Periodc (days) Coursed Invasione (% Infected RBCs) IIFf ELISAg
Vaccinea # Sex Species
Freund’s alone 701 Male N 9 Virulent 1.5 <102 <102
886 Female N 12 Virulent 1.7 <102 <102
184 Male V 7 Virulent 1.3 <102 <102
yMSP119 331A Male N 11 Self-resolved 1.6 104 106
218 Female N 12 Self-resolved 1.6 104 105
1193 Male V 28 Virulent 1.7 >105 106
2458 Male V 11 Virulent 2.1 104 106
bGST-MSP142 202 Male N 10 Virulent ND 103 103
E851 Male N 11 Persistent ND 103 104
T212 Male V 8 Virulent ND 104 104
  1. ayMSP119, yeast produced fusion of the 19-kD C-terminus of MSP119; GST-MSP142, bacterial produced 42-kD C-terminus of MSP1 fused with glutathione-S-transferase.
  2. bSpecies of Aotus: N, A. nancymai; V, A. vociferans.
  3. c Prepatent period: time from inoculation of parasites to first visualized parasite in the blood.
  4. dVirulent, rose to above 5%, requiring treatment; self-resolved, after a low-grade parasitemia of <0.1%; Persistent, no control, but parasitemia always below 5%.
  5. ePercentage of red cells that contained ring-stage parasites in the invasion assay performed in the presence of Aotus sera from Day 17 after the third immunization (the day of parasite challenge).
  6. fIIF, indirect immunofluorescence against FCRIII that has the identical C-terminus as the FVO strain of P. falciparum.
  7. gELISA, enzyme-linked immunosorbent assay. End-point titers defined as the highest dilution of sera with an A405 of 0.4 or greater (data from three different assays).