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Fig. 2 | Molecular Medicine

Fig. 2

From: Association Between HLA DQBl * 03 and Cervical Intra-epithelial Neoplasia

Fig. 2

A 4% agarose gel showing amplified DNA after PCR with primers A and B with and without digestion by Mlu I

The size of amplified DNA is 145 bp and on digestion with Mlu I, a 115 and 30 bp product is produced in DQB1 * 03 homozygotes. DNA for all controls were from the British Society for Histocompatibilty and Immunogenetics. Arrows show the 145 and 115 bp products. (Lanes 2 and 3) Heterozygous DQB1 * 03 control with and without digestion by Mlu I. (Lanes 4 and 5) Homozygous DQB1 * 03 control with and without digestion with Mlu I. (Lanes 6 and 7) Non-DQB1 * 03 control with and without digestion with Mlu I. (Lanes 1 and 8) 123 bp markers.

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