|
Patients (No.)
|
HLA DQB1 * 03 (Positive) (%)
|
Odds Ratio (95% CI)
|
|---|
CIN (178)
CIN 1 (66) CIN 3 (112)
|
109 (61) 37 (56) 72 (64)
|
3.03 (2.11–4.35)
2.45 (1.45–4.12) 3.45 (2.23–5.33)
|
|
HPV negative CIN (47)
|
25 (53)
|
2.18 (1.19–3.97)
|
|
HPV positive CIN (131)
|
84 (64)
|
3.43 (2.28–5.15)
|
|
Controls (420) (HPV negative
|
144 (34)
|
1*
|
- HLA DQB1 * 03 typing was performed on DNA from cervical smears of 178 women with CIN (CIN I = 66; CIN III = 112) and 420 healthy women who had a normal smear. All samples were successfully amplified for the locus. HPV typing was performed for types 16, 18, 31, and 33 on all the test and control samples.
- Of CIN cases, 61% were positive (56% of CIN 1, 64% of CIN III) for the HLA DQB1 * 03 type, compared with 34% of controls. This was significant (χ2 trend = 37.3, p < 0.001), and the odds ratio for CIN overall was 3.03 (95% CI 2.11–4.35). The association was significant for both CIN III and CIN I (odds ratio 3.45 versus 2.45), stronger for CIN HI, but not significantly different from CIN I.
- One hundred thirty-one patients with CIN (73.5%) were positive for one or more HPV types 16, 18, 31, and 33. Of HPV-positive CIN, 64% were of the type DQB1 * 03. There was a significant association between DQB1 * 03 and HPV (χ2 trend = 23.2, p < 0.001) with an odds ratio of 3.43 (95% CI 2.28–5.15).
