|
HLA DQB1 * 03 Allele
|
CIN 3 (2n = 224)
|
CIN 1 (2n = 132)
|
Controls (2n = 840)
|
Odds Ratio (95% CI)
|
χ2 Trend
|
|---|
|
0301
|
49 (21)
|
25 (19)
|
79 (9)
|
2.53 (1.79–3.57)
|
28.6 (p < 0.001)
|
|
0302
|
45 (20)
|
16 (12)
|
85 (10)
|
1.84 (1.29–2.62)
|
15.5 (p < 0.001)
|
|
0303
|
5 (2)
|
7 (5)
|
21 (2.5)
|
1.36 (0.67–2.76)
|
0.05 (p = 0.82)
|
|
0304
|
0
|
0
|
1 (0.1)
|
0
| |
|
Non-DQB1 * 03
|
125
|
84
|
654
|
1*
| |
|
Total
|
224
|
132
|
840
| | |
- The ARFLP-PCR technique used on DNA from cervical smears, following Mlu I digestion, identifies women who are negative, heterozygous, or homozygous for the DQB1 * 03 locus (Fig. 2). Of women with CIN, 38% were negative, 37% were heterozygous, and 23% were homozygous for the DQB1 * 03 locus (χ2 trend = 39.01, p < 0.001). Compared with controls, the odds ratio was greater for homozygosity (4.0, 95% CI 2.43–6.6) than for heterozygosity (2.63, 95% CI 1.75–3.94). Further typing of the DQB1 * 03 locus in positive samples showed that the 0301 allele was present in 40% of CIN as opposed to 9% of controls (odds ratio 2.53, 95% CI 1.79–3.57; χ2 trend = 28.6, p <0.001). DQB1 * 0302 was present in 32% and 10% of CIN and controls, respectively (odds ratio 1.84, 95% CI 1.29–2.62).
