Fig. 2From: Adenovirus-Mediated Transfer of the Herpes Simplex Virus Thymidine Kinase Gene Inhibits Vascular Smooth Muscle Cell Proliferation and Neointima Formation Following Balloon Angioplasty of the Rat Carotid Artery Inhibition of neointimal formation by ADV-tk/+GC following arterial injury (opposite) Adult rats underwent carotid artery angioplasty and adenovirus-mediated gene transfer with ADV-tk or ADV-ΔE1 as described in Materials and Methods, and were then randomized to receive systemic therapy with either ganciclovir (ADV-tk/ + GC or ADV-ΔE1/ + GC) or saline (ADV-tk/-GC or ADV-ΔE1/-GC) for 6 days. Carotid arteries were obtained for analysis 20 days following arterial injury and gene transfer. Representative photomicrographs from ADV-ΔE1/-GC (A and B), ADV-ΔE1/ + GC (C and D), ADV-tk/-GC (E and F), or ADV-tk/ + GC (G and H) treated arteries are shown. (A, C, E, and G) Magnification 100×; (B, D, F, and H) 400×. Arrows delineate the internal elastic lamina. Note the significant reduction in neointima formation in the ADV-tk/ + GC artery.Back to article page