Fig. 2From: Hypoxic Induction of Endothelial Cell Growth Factors in Retinal Cells: Identification and Characterization of Vascular Endothelial Growth Factor (VEGF) as the Mitogen Effect of hypoxia on steady state levels of VEGF and bFGF mRNA hRPE were plated at 15,000 cells/cm2 and allowed to grow to confluence. Total RNA was isolated from hRPE grown under normoxic or hypoxic conditions for 6, 12, and 24 hr. After probing for VEGF (a) the same blot was stripped and used for analysis of bFGF (b) and 28S rRNA (c).Back to article page