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Fig. 3 | Molecular Medicine

Fig. 3

From: Hypoxic Induction of Endothelial Cell Growth Factors in Retinal Cells: Identification and Characterization of Vascular Endothelial Growth Factor (VEGF) as the Mitogen

Fig. 3

RT-PCR and Southern blot analysis of mRNA from normoxic and hypoxic hRPE

(a) hRPE were cultured as described in the legend of Fig. 2. RT-PCR (35 cycles) of total RNA from normoxic (Lanes 1 and 2) and hypoxic (Lanes 3 and 4) hRPE, visualized by ethidium bromide-stained agarose gel analysis. M1, 123 bp ladder, M2 HaeIII digest of phi X174; C, is a control using monkey retina mRNA, which contains both VEGF121 and VEGF165 splice variants. (b) Southern blot analysis of RT-PCR products using a hVEGF cDNA probe. Products from cycles 25 and 30 were analyzed for normoxic cDNA (Lanes 1 and 4), hypoxic cDNA (lanes 2 and 5) and control monkey retina cDNA’s (Lanes 3 and 6). Blank cDNA controls yielded no detectable products (data not shown).

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