Effect of hypoxia on the synthesis of VEGF and bFGF
hRPE were cultured as described in the legend of Fig. 2. At confluence the hRPE were grown for 24 hr in normoxic and hypoxic environments and 35S-labeled proteins were immunoprecipitated from conditioned media and cell lysates using sheep anti-human recombinant-bFGF IgG (Lanes 1 and 3) or rabbit antisera to VEGF (Lanes 2 and 4). Controls included sheep non-immune IgG (Lane 5) and control nonimmune rabbit serum (Lane 6). Specificity of the VEGF antisera is demonstrated by competition for 35S-VEGF in the conditioned media (upper right panel).