Fig. 5

Effect of hypoxia on steady-state VEGF and bFGF in hRPE

Conditioned media levels of (a) VEGF and (b) bFGF and cell lysates levels of (c) VEGF and (d) bFGF in sparse and confluent culture of hRPE were quantified by immunoassay. Open bars indicate cells grown under normoxia and black bars indicate hypoxic exposure. Levels of growth factor were normalized to cell number obtained from parallel cultures and are expressed as pm/10⁶ cells ± SEM.