Expression Cloning of cDNAs That Render Cancer Cells Resistant to Pseudomonas and Diphtheria Toxin and Immunotoxins

Table 1 Plasmid amplification by selection-cloning results in siblings with the same or similar cDNA insert

Ninety-six plasmid clones from the third round of selection were hybridized with inserts of 10 randomly chosen clones that were labeled with digoxigenin as described in Materials and Methods. Six of these inserts hybridize not only with themselves but also with other plasmids ([+1 hybridization signal; [−] no signal). Preliminary sequencing data (not shown) indicate that some hybridizing clones are identical siblings and others nonidentical clones with related sequences.

ISSN: 1528-3658