Skip to main content

Table 1 Plasmid amplification by selection-cloning results in siblings with the same or similar cDNA insert

From: Expression Cloning of cDNAs That Render Cancer Cells Resistant to Pseudomonas and Diphtheria Toxin and Immunotoxins

Hybridization Probe
Plasmid 90 25 30 17 50 63 31 33 68 99
p90 + + +
p25 + + +
p30 + + +
p35 + +
p96 + +
p55 + +
p17 +
p50 +
p28 +
p40 +
p63 +
p60 +
p81 +
p31 +
p33 +
p68 +
p99 +
  1. Ninety-six plasmid clones from the third round of selection were hybridized with inserts of 10 randomly chosen clones that were labeled with digoxigenin as described in Materials and Methods. Six of these inserts hybridize not only with themselves but also with other plasmids ([+1 hybridization signal; [−] no signal). Preliminary sequencing data (not shown) indicate that some hybridizing clones are identical siblings and others nonidentical clones with related sequences.