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Table 2 Viability of MCF-7 transfectants after toxin exposure

From: Expression Cloning of cDNAs That Render Cancer Cells Resistant to Pseudomonas and Diphtheria Toxin and Immunotoxins

PE (ng/ml) cfu After a 48-hr Exposure to PE
MCF-7/N MCF-7/17 MCF-7/30 MCF-7/50 MCF-7/90*
0 200,000 200,000 200,000 200,000 200,000
3 4,500 50,000 20,000 35,000 30,000
10 250 9,000 10,000 20,000 15,000
30 0 200 150 250 100
  1. A hundred thousand cells in 24-well plates were incubated 48 hr with toxin, washed twice with PBS, trypsinized, and plated at serial dilutions in medium without toxin.
  2. Colonies were visualized 6 days after plating by methylene-blue staining, and the colony numbers (cfu) calculated by the colonies/well × dilution factor. *MCF-7/90 was treated with identical conditions but in a separate experiment.