PE (ng/ml) | cfu After a 48-hr Exposure to PE |
|---|
MCF-7/N | MCF-7/17 | MCF-7/30 | MCF-7/50 | MCF-7/90* |
|---|
0 | 200,000 | 200,000 | 200,000 | 200,000 | 200,000 |
3 | 4,500 | 50,000 | 20,000 | 35,000 | 30,000 |
10 | 250 | 9,000 | 10,000 | 20,000 | 15,000 |
30 | 0 | 200 | 150 | 250 | 100 |
- A hundred thousand cells in 24-well plates were incubated 48 hr with toxin, washed twice with PBS, trypsinized, and plated at serial dilutions in medium without toxin.
- Colonies were visualized 6 days after plating by methylene-blue staining, and the colony numbers (cfu) calculated by the colonies/well × dilution factor. *MCF-7/90 was treated with identical conditions but in a separate experiment.
