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Table 3 Sensitivity of MCF-7 and transfectants towards toxins that inhibit protein synthesis

From: Expression Cloning of cDNAs That Render Cancer Cells Resistant to Pseudomonas and Diphtheria Toxin and Immunotoxins

Cell Line IC50 (ng/ml)
IT PE DT RC CY
MCF-7/N 1.5 1.0 0.006 0.027 300
MCF-7/17 15 15 0.07 0.02 220
MCF-7/30 10 15 0.1 0.027 220
MCF-7/50 6 5 0.1 0.024 200
MCF-7/90 7 10 0.04 0.024 220
  1. The toxicity of the immunotoxin B3(Fv)-PE38KDEL (IT), Pseudomonas exotoxin (PE), Diphtheria toxin (DT), ricin (RC), and cycloheximide (CY) was determined by MTS assays (24) (see Materials and Methods). IC50 is the concentration that reduces the signal to 50% of cells that were not incubated with toxin.