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Table III Differential Heat Inactivation of Enzymes in Dog and Rabbit Serum Which Destroy the Transforming Substance

From: Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types

    Triplicate tests
  Heat treatment of serum Dilution* 1 2 3
    Diffuse growth Colony form Diffuse growth Colony form Diffuse growth Colony form
Dog serum Unheated Undiluted R only R only R only
1:5 R “ R “ R “
1:25 R “ R “ R “
60°C. for 30 min. Undiluted + SIII + SIII + SIII
1:5 + SIII + SIII + SIII
1:25 + SIII + SIII + SIII
65°C. for 30 min. Undiluted + SIII + SIII + SIII
1:5 + SIII + SIII + SIII
1:25 + SIII + SIII + SIII
Rabbit serum Unheated Undiluted R only R only R only
1:5 R “ R “ R “
1:25 R “ R “ R “
60°C. for 30 min. Undiluted R only R only R only
1:5 R “ R “ R “
1:25 R “ R “ R “
65°C. for 30 min. Undiluted + SIII + SIII + SIII
1:5 + SIII + SIII + SIII
1:25 + SIII + SIII + SIII
Control (no serum) None Undiluted + SIII + SIII + SIII
1:5 + SIII + SIII + SIII
1:25 + SIII + SIII + SIII
  1. * Dilution of the digest mixture of serum and transforming substance.