Table III Differential Heat Inactivation of Enzymes in Dog and Rabbit Serum Which Destroy the Transforming Substance
From: Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types
| Â | Â | Â | Triplicate tests | |||||
|---|---|---|---|---|---|---|---|---|
| Â | Heat treatment of serum | Dilution* | 1 | 2 | 3 | |||
| Â | Â | Â | Diffuse growth | Colony form | Diffuse growth | Colony form | Diffuse growth | Colony form |
Dog serum | Unheated | Undiluted | − | R only | − | R only | − | R only |
1:5 | − | R “ | − | R “ | − | R “ | ||
1:25 | − | R “ | − | R “ | − | R “ | ||
60°C. for 30 min. | Undiluted | + | SIII | + | SIII | + | SIII | |
1:5 | + | SIII | + | SIII | + | SIII | ||
1:25 | + | SIII | + | SIII | + | SIII | ||
65°C. for 30 min. | Undiluted | + | SIII | + | SIII | + | SIII | |
1:5 | + | SIII | + | SIII | + | SIII | ||
1:25 | + | SIII | + | SIII | + | SIII | ||
Rabbit serum | Unheated | Undiluted | − | R only | − | R only | − | R only |
1:5 | − | R “ | − | R “ | − | R “ | ||
1:25 | − | R “ | − | R “ | − | R “ | ||
60°C. for 30 min. | Undiluted | − | R only | − | R only | − | R only | |
1:5 | − | R “ | − | R “ | − | R “ | ||
1:25 | − | R “ | − | R “ | − | R “ | ||
65°C. for 30 min. | Undiluted | + | SIII | + | SIII | + | SIII | |
1:5 | + | SIII | + | SIII | + | SIII | ||
1:25 | + | SIII | + | SIII | + | SIII | ||
Control (no serum) | None | Undiluted | + | SIII | + | SIII | + | SIII |
1:5 | + | SIII | + | SIII | + | SIII | ||
1:25 | + | SIII | + | SIII | + | SIII | ||