Immunohistochemical detection of huntingtin in HD brain
(A) Huntingtin expression. A cryostat section of frontal cortex from an HD grade 3 postmortem brain was immunostained for huntingtin using a 1:1000 dilution of HF1 immune serum (630×). The section is counterstained with hematoxylin. (B) Detection of huntingtin is abolished by preincubation with antigen. A section of HD frontal cortex nearly adjacent to that shown in Panel A was immunostained with HF1 immune sera that had been preabsorbed with 12 µg/ml of HF1 fusion protein (as in Fig. 1B) (630×). The section is counterstained with hematoxylin.