Fig. 2From: Thalidomide Treatment Reduces Tumor Necrosis Factor α Production and Enhances Weight Gain in Patients with Pulmonary Tuberculosis The effect of thalidomide treatment on TNFα production in vitro TNFα levels were evaluated by ELISA in the supernatants of PBMCs obtained from 12 HIV-1− and 10 HIV-1+ patients treated with MDT and 300 mg/day of thalidomide (left) and eight patients (5 HIV-1−) treated with MDT and placebo (right). PBMCs obtained before (Pre) on Day 7 of treatment (Mid) and 5 days after (Post) the first cycle of treatment were stimulated with LPS and culture supernatants were collected for assay. Results are means ± one SEM for samples obtained from all patients tested. TNFα levels were significantly lower during treatment with thalidomide relative to levels before (p = 0.001) or after (p = 0.002) thalidomide.Back to article page