Northern blot analyses of TIMP3 mRNAs using (A) 500 ng polyA+ RNA or (B) 8 µg total RNA from human tissues and human cell lines
Panel A indicates that TIMP3 mRNAs were present at higher levels in the breast carcinoma (BC) tissue used both in cDNA library and differential probe construction than in the fibroadenoma (FA) used to subtract breast carcinoma cDNAs. Panel B indicates that while 4.6-, 2.7-, and 2.5-kb TIMP3 transcripts (corresponding to cDNA clones S6/S10, S5, and S4, respectively) were detected in HFL1 fibroblasts, the 4.6-kb TIMP3 transcript was not detected in HeLa and Caco-2 cell lines. Blots were rehybridized with 36B4 cDNA (25), corresponding to a gene ubiquitously expressed. Autoradiographic exposures were for 19 and 6 hr for TIMP3 and 36B4, respectively (A), and for 3 days for both TIMP3 and 36B4 (B).