Structures of the human TIMP3 transcripts
The 5′-UTR, coding sequence, and 3′-UTR of the 4.6-kb human TIMP3 transcript are illustrated diagrammatically at the top of the figure; the vertical lines through the 3′-UTR indicate the positions of functional polyadenylation signals identified by cloning the S1, S4, S5, S6, and S10 (S6/10) cDNAs. The sequence of each polyadenylation signal is indicated above a horizontal line representing the predicted length of the TIMP3 transcript relative to the 4.6-kb TIMP3 sequence. Note that the 3′-UTR occupies 56–80% of the four TIMP3 transcripts shown. The TIMP3 2.1-kb transcript in the present study was previously reported as a 1.8 kb transcript (21). The S6/10 sequence, annotated so as to indicate the positions of the 4 polyadenylation signals identified, has been deposited in the GenBank database under the accession number U14394.