Activation of p56 (top band) and p53 (bottom band)
kinase autophosphorylation by LPS
C3H/OuJ (Lpsn) macrophages were treated with medium only (resting cell kinase level), or stimulated with 100 ng/ml LPS. Lane 1, unstimulated cells immunoprecipitated with an irrelevant antibody; Lanes 2–6 were immunoprecipitated with anti-lyn antibody. Lane 2, unstimulated cells (resting cell kinase level); lane 3, 0.083 min; Lane 4, 5 min; Lane 5, 10 min; Lane 6, 15 min; and Lane 7, 30 min after LPS stimulation. Lyn kinase autophosphorylation was measured in the in vitro kinase assay as described in Materials and Methods by incorporation of 32P. This autoradiogram is derived from a single representative experiment.