Western blot analysis of p56 and p53
protein in cell lysates used for immunoprecipitation in the kinase assay shown in Fig.
Equivalent volumes of sample lysates were processed for Western blot analysis as described in Materials and Methods. Lanes 1 and 2, unstimulated cells; Lane 3, 0.083 min; Lane 4, 5 min; Lane 5, 10 min; Lane 6, 15 min; and Lane 7, 30 min after LPS stimulation. Molecular weights were calculated based on prestained protein standards.