Fig. 1

Rabbit aortic tissue (A) and serum (B) AGE levels after intravenous administration of AGE-modified rabbit serum albumin (AGE-RSA)

Following treatment with either AGE-RSA (16 mg/kg/day, i.v.) (AGE) alone for 4 months, 2% USP cholesterol-rich diet (CRD) supplemented with 10% corn oil for 2 weeks, normal saline control (0.5 ml/day, i.v.) (CL), or AGE for 4 months plus CRD for last 2 weeks (AGE + CRD), AGE levels were determined in collagenase-digested aortic tissue extracts using an AGE-specific ELISA. (A) Aorta AGE levels (mean ± SEM, n = 5/group). Significant p values: AGE versus CL, < 0.025, AGE versus CRD < 0.025; AGE + CRD versus CL < 0.01, AGE + CRD versus CRD < 0.001. (B) Serum AGE levels (mean ± SEM, n = 5/group) p values: AGE versus CL and CRD < 0.005 each; AGE + CRD versus CL and CRD < 0.025 each.