Fig. 1
From: Taxol-Induced Mitotic Block Triggers Rapid Onset of a p53-Independent Apoptotic Pathway
Kinetics of taxol-induced block in mitosis and cytotoxicity in HeLa cells
Cells were treated with 1 µM (◇), 100 nM (▲), 25 nM (●), 10 nM (▽), or 0 (□) taxol and scored at intervals for arrest in prophase by Hoechst staining (solid lines) or cell death by Trypan blue staining (dashed lines). Both attached and detached cells were scored, since cells arrested in prophase soon detach, and summed to give the total cell number. The ratio of cell numbers attached:detached was used to calculate overall percentage of cells in M.