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Fig. 3 | Molecular Medicine

Fig. 3

From: Taxol-Induced Mitotic Block Triggers Rapid Onset of a p53-Independent Apoptotic Pathway

Fig. 3

Sequence of changes induced by taxol in HeLa cells

Untreated (A and B) and taxol-treated (4 hr; C and D) cells processed for anti-tubulin IF (B and D) and counterstained with Hoechst 33342 nuclear dye (A and C) to reveal multipolar spindles induced by taxol. A few cells undergo aberrant mitosis exhibiting irregular chromosome arrays at metaphase (I). After 24-hr taxol treatment, Hoechst staining reveals DNA becomes globular (E and G Hoechst staining; F and H corresponding phase) especially when cells have extensive membrane blebbing (H). Exposure times for Panels E and G were shorter than for Panels A, C, and I. Bar in Panel I represents 10 µm; magnification is the same for all panels.

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