Fig. 5
From: Taxol-Induced Mitotic Block Triggers Rapid Onset of a p53-Independent Apoptotic Pathway
TUNEL staining for in situ identification of apoptotic cells reveals that DNA nicking is detected first in taxol-treated cells blocked in prophase.
HeLa cells grown on coverslips in normal medium (A–C, I and J) or treated with 100 nM taxol for 8 hr (D–F) or 100 nM etoposide for 3 days (G and H), as well as multimininucleated G1-arrested Hs578T cells remaining attached after 4 days following 25 nM taxol treatment (K and L) were processed for TUNEL labeling of nicked DNA (C, F, H, J, and L) and counterstained with Hoechst 33342 (B, E, G, I, and K). Cells in Panels I and J were treated with micrococcal nuclease for 20 min after fixation and prior to TUNEL labeling. Bar in Panel A represents 10 µm; magnification is the same for all panels.