Fig. 6
From: Taxol-Induced Mitotic Block Triggers Rapid Onset of a p53-Independent Apoptotic Pathway
Time course for onset of block in prophase (o) and appearance of TUNEL positive cells (â–²) in cell-cycle synchronized cell cultures.
TUNEL staining occurred exclusively in cells blocked in prophase. Cells were plated onto coverslips immediately after shake-off (60% cells in mitosis by Hoechst staining). After 12 hr, 200 nM taxol was added and coverslips collected at the indicated time points, fixed, processed for TUNEL, and counter-stained with DAPI. Two sets of 250 mitotic cells were scored concurrently for arrest in prophase and TUNEL staining.