Fig. 1

PCR analysis of V δ 2-J δ 1 and V δ 2-J δ 3 in freshly isolated PBMC, or in cells cultured with PPD and selected for reactivity to HSP70

In Panel A, lanes from the left contain molecular weight markers followed by PCR analysis for Vδ2-Jδ1, Vδ2-Jδ3, and β-actin for one MS patient, one healthy control, and one TB patient, respectively, for PPD⁺HSP70⁺ T cell lines. In Panel B, lanes from the left contain molecular weight markers followed by PCR analysis for one healthy control for Vδ2-Jδ1, Vδ2-Jδ3, and β-actin for freshly isolated PBMC cultured alone, PPD⁺HSP70⁺ and PPD⁺HSP70⁻ T cell lines.