Sample | Vδ2 | P/N | Dδ | N | Jδ3 |
---|
germ-line | TGT GAC ACC | | | | C TCC TGG GAC AC |
| | | δ1 GAAATAGT | | |
| | | δ2 CCTTCCTAC | | |
| | | δ3 ACTGGGGGATACG | | |
PPD+HSP70+ | | | | | |
CONT #1#2 | TGT GAC ACC | GTG | CTG GGG GAT ACG | NNN G | CC TGG GAC AC |
| C D T | V | L G D T | (*) | A W D T |
PPD+HSP70+ | | | | | |
MS #1,2,3,4 | TGT GAC ACC | GTC | GGA | CAC C | AC AC |
TB #2, #3 | C D T | V | G | H | H T |
PPD+HSP70+ | TGT GAC A | GC CGT | TGG G | CA CAC GCA | TCC TGG GAC AC |
TB #1 | C D | S R | W | A H A | S W D T |
PPD+HSP70− | TGT GAC ACC | G | CT GGG GGG | CG | C TCC TGG GAC AC |
TB #1 | C D T | | A G G | R | S W D T |
- Vδ2-Jδ3 junctional sequences from direct sequence gels of PCR products from freshly isolated PBMC or cells cultured with PPD and tested for reactivity to HSP70. Sequences obtained from HSP70+ T cell lines from controls 1 and 2 differed from one another by only a short region of heterogeneity. Sequences obtained from HSP70+ T cell lines from MS patients 2, 3 and 4, and TB patients 2 and 3 were identical. Sequences obtained from an HSP70+ and an HSP70− T cell line from one TB patient were oligoclonal but differed from all other sequences.