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Table 2 Nucleotide and deduced amino acid sequences detected by direct sequencing

From: γδ T Cell Receptor Analysis Supports a Role for HSP 70 Selection of Lymphocytes in Multiple Sclerosis Lesions

Sample

Vδ2

P/N

Dδ

N

Jδ3

germ-line

TGT GAC ACC

   

C TCC TGG GAC AC

   

δ1 GAAATAGT

  
   

δ2 CCTTCCTAC

  
   

δ3 ACTGGGGGATACG

  

PPD+HSP70+

     

CONT #1#2

TGT GAC ACC

GTG

CTG GGG GAT ACG

NNN G

CC TGG GAC AC

 

C D T

V

L G D T

(*)

A W D T

PPD+HSP70+

     

MS #1,2,3,4

TGT GAC ACC

GTC

GGA

CAC C

AC AC

TB #2, #3

C D T

V

G

H

H T

PPD+HSP70+

TGT GAC A

GC CGT

TGG G

CA CAC GCA

TCC TGG GAC AC

TB #1

C D

S R

W

A H A

S W D T

PPD+HSP70

TGT GAC ACC

G

CT GGG GGG

CG

C TCC TGG GAC AC

TB #1

C D T

 

A G G

R

S W D T

  1. Vδ2-Jδ3 junctional sequences from direct sequence gels of PCR products from freshly isolated PBMC or cells cultured with PPD and tested for reactivity to HSP70. Sequences obtained from HSP70+ T cell lines from controls 1 and 2 differed from one another by only a short region of heterogeneity. Sequences obtained from HSP70+ T cell lines from MS patients 2, 3 and 4, and TB patients 2 and 3 were identical. Sequences obtained from an HSP70+ and an HSP70 T cell line from one TB patient were oligoclonal but differed from all other sequences.