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Fig. 4 | Molecular Medicine

Fig. 4

From: Ciliary Neurotrophic Factor Inhibits Brain and Peripheral Tumor Necrosis Factor Production and, When Coadministered with Its Soluble Receptor, Protects Mice From Lipopolysaccharide Toxicity

Fig. 4

CNTF potentiates DEX inhibition of TNF production in vitro

Mouse spleen cells were cultured 4 hr as described in Materials and Methods with 1 µg/ml LPS, 10 µg/ml CNTF, and 20 µg/ml sCNTFRα. No TNF was detected in the absence of LPS (data not shown). DEX was added at various concentrations (0 or 10−3 to 10−9 M) in the presence and absence of CNTF (10 µg/ml). Curve fitting was performed by the Allfit program (23). (○), LPS alone; (□), LPS + CNTF; (*), LPS + CNTF + sCNTFRα. TNF production in the absence of DEX was: 1570 ± 479 for LPS alone; 1007 ± 308 pg/ml (mean ± SD; n = 3) for LPS with CNTF.

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Molecular Medicine

ISSN: 1528-3658