| | Single Clonal Sequencea | | Mixed Sequencesb | |
|---|
Counts in Dominant Band | CB-R (%) | CB-R or BJ-R (%) | 1 nt. Position (%) | 2 nt. Positions (%) | 3 nt. Positions (%) | >3 nt. Positions (%) |
|---|
≧50% (n = 34) | 47 | 82 | 3 | 3 | 3 | 9 |
45–50% (n = 15) | 6 | 67 | 12 | 0 | 12 | 6 |
- aDominant bands containing ≧50% and 45–50% of the counts within a CD8+ TCR-specific PCR product were sequenced directly with CB-R and/or specific BJ-R primers to establish oligoclonality. The sequence success rate was determined on the basis of cleanly readable dominant sequence with no discrepancies at all.
- bThe percentage of those dominant bands containing ≧50% and 45–50% of the counts in which direct sequencing with CB-R and/or BJ-R primers yielded sequences with discrepancies at 1, 2, 3, or >3 nucleotide (nt.) positions.
