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Fig. 2 | Molecular Medicine

Fig. 2

From: Identification of Galectin-3 As a High-Affinity Binding Protein for Advanced Glycation End Products (AGE): A New Member of the AGE-Receptor Complex

Fig. 2

Ligand blot analysis of galectins 1–4

Recombinant human galectin-1, -2, -3 and rat galectin-4 (domain I) were subjected to SDS-PAGE (4–20% gel, 5 µg/lane) and electroblotted onto nitrocellulose membrane. (A) Blots were either stained with Amido black or probed with 125I-AGE-BSA. Protein staining of filters (1) and autoradiographs of ligand blots (2) are shown. (B) Comparison of 125I-AGE-BSA binding to galectins by phosphorimage (PI) analysis. Data are expressed in relative PI units/µg protein.

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Molecular Medicine

ISSN: 1528-3658