Fig. 2
From: H-RYK, an Unusual Receptor Kinase: Isolation and Analysis of Expression in Ovarian Cancer
Two partial metaphase spreads showing localization of probe to human chromosome 3q24 (arrowed)
Signal was detected with Texas red on R-banded chromosomes stained with anti-bromodeoxyuridine-FITC. DAPI counterstaining of the same spreads produced a G-banding pattern. Metaphase spreads were processed using a Zeiss Axioscop microscope equipped with a CCD camera (Photometrics, Photometrics, AZ, U.S.A.). Separate images of probe signal, banding pattern, and counterstain were captured and colored. These images were then merged using a computer software developed by T. Rand and D. C. Ward (Yale University, New Haven, CT).