FIG. 6
From: H-RYK, an Unusual Receptor Kinase: Isolation and Analysis of Expression in Ovarian Cancer
Western blot analyses of 41M and CHO cells
(a) Western blot analysis of 41M cell line using 15.1 and 15.2 antibodies. The markers are from Amersham and are expressed in kilodaltons. I.P., immunoprecipitation; W.B., Western blot. Two bands around 116 kD are identified specifically by either 15.1 or 15.2 in the absence of cognate peptides. (b) Western blot analysis of 41M cell lines using 15.1 and 15.2 antibodies with a longer exposure. An additional band is observed around 45 kD in the absence of cognate peptides in Lanes 2 and 3. (c) Western blot analysis of CHO cells transiently transfected with H-RYK. Immunoprecipitation was performed with 15.2 in the presence or absence of cognate peptides. Blotting was performed with the same antibody. A specific band around 100 kD is detected by 15.2 in transfected cells. Protein markers are from Amersham and are expressed in kilodaltons.