Fig. 1

Effect of a near-lethal dose of endotoxin on PTHrP mRNA levels in vital organs

Male Sprague Dawley rats were injected via tail vein with 5 mg/250 g body weight Escherichia coli strain 055:B5 LPS. At indicated times, spleen (A; circle), lungs (A; triangle), heart (B), kidneys (C; circle), and liver (C; triangle) were removed for Northern analysis of polyadenylated mRNA using a murine PTHrP cDNA probe (4). PTHrP mRNA is reported as mean ± SEM (n = 4/time). Differences are statistical significant by ANOVA; p values determined by two-tailed Student’s t test. *p < 0.05 (versus t = 0); **p < 0.01; ***p < 0.005; ****p < 0.001.