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Fig. 6 | Molecular Medicine

Fig. 6

From: Vascular Gene Transfer of the Human Inducible Nitric Oxide Synthase: Characterization of Activity and Effects on Myointimal Hyperplasia

Fig. 6

Characterization of iNOS expression and activity in transduced RSMCs

(A) \({\rm{NO}}_2^ - \) production as measured by the Griess reaction in uninfected, BaglacZ, and DFGiNOS RSMCs. Each bar represents the mean ± SD (n = 3, each experiment repeated three times). iNOS activity was measured ±L-NMA (0.5 mM), ±BH4(100 µM). *p < 0.01 versus uninfected and BaglacZ cells by ANOVA. (B) Northern blot analysis for human iNOS mRNA in RSMC, RSMC-BaglacZ, and RSMC-DFGiNOS cells. A 7.5-kb iNOS signal was detected in total RNA from RSMC-DFGiNOS only. The endogenous human hepatocyte iNOS mRNA from cytokine stimulated human hepatocytes (Hum HC + CM, 6 hr) measures 4.5 kb.

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