Fig. 1

Comparative sequence of the mouse and human iNOS promoter regions and identification of consensus promoter response elements

The sequence of the entire 3814-bp human iNOS promoter region cloned and sequenced is presented. The previously published murine sequence is presented as the lower series of rows (GenBank accession numbers L23806 and L09126). The transcriptional start site, indicated by lower case letters, was identified from Ref. 12. The differences in the sequences discussed in the text are presented in bold. Promoter response elements were identified using the consensus sequences cited in Table 1, and the position and orientation are identified by arrows under the indicated regions. Only exact matches, or matches as previously reported are shown. Alignment matching of the murine and human sequences was determined by using the NALIGN program of the PCGene package (Intelligenetics. Mountain View, CA, U.S.A.) using the default values for weighting open gaps as 10 and unit gap costs as 10.