Fig. 2
From: Characterization and Functional Analysis of the Human Inducible Nitric Oxide Synthase Gene Promoter
Murine and human iNOS promoter activity in both human and mouse cells
RAW 267.4, and A549 cells were cultured as described. Transfections were performed as described in Materials and Methods. Equal numbers of cells (1 × 106) were used in all cases. Luciferase activity is presented as percent increase over that present in nonactivated cells. RAW 267.4 cells were stimulated with LPS (10 µg/ml, solid bar), or nonstimulated (open bar). A549 were transfected with promoter/reporter constructs, incubated for 24 hr and then activated with IL-1β (100 µ/ml), IFNγ (500 µ/ml), and TNFα (10 ng/ml) for 8 hr (solid bars) or non-stimulated (open bars).