Fig. 4From: Characterization and Functional Analysis of the Human Inducible Nitric Oxide Synthase Gene Promoter Activation of the mouse 1.5-kb and human 1.8-kb promoter regions transfected into human A549 cells stimulated with single or multiple cytokines Luciferase activity was quantified after the transfection of either the murine 1.5-kb promoter/reporter construct (open bars) or the human analog (solid bars) into equal numbers of A549 cells followed by 8 hr of activation with the indicated cytokines (100 µ/ml IL-1β, 10 ng/ml TNFα, or 500 µ/ml of IFNγ alone, or in combinations as indicated). Data are presented as the percentage increase over that present in nonactivated cells.Back to article page