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Table 1 Frequency of promoter response elements in the human 3.8-and 1.8-kb and mouse 1.5-kb promoter regions

From: Characterization and Functional Analysis of the Human Inducible Nitric Oxide Synthase Gene Promoter

Element

Consensus Sequence

Ref.

n/n a

Human 3.8-kb Clone b

Human 1.8-kb Clone c

Mouse 1.5-kb Clone d

γ IRE

CTKKANNYe

24

8/8

11+

8−

9+

1−

4+

0−

   

7/8

141+

140−

50+

52−

5+

1−

AP-1

TGACTCA

25

7/7

0+

0−

    
   

6/7

5+

4−

3+

1−

1+

1−

NF-IL6

AGTTANGNAA

26

10/10

1+

0−

1+

0−

  
   

9/10

4+

2−

3+

1−

  

NF-κB

GGGRNNYYCC

27

10/10

1+

0−

1+

0−

2+

0−

TNF-RE

TGAGCTCA

28

8/8

0+

0−

    
   

7/8

5+

n/a

3+

n/a

2+

n/a

X-box

CCYAGMRACNG

24

8/11

0+

0−

  

1+

0−

GAS

TTACTCTAAA

24

8/10

0+

0−

  

1+

0−

IRF-E

SYAAAGYSAAA(A) G

29

11/13

1+

0−

0+

0−

0+

0−

   

10/13

0+

2−

0+

2−

  

ISRE

YAGTTTC (A/T) YTTTCC

30

11/14

2+

2−

1+

2−

2+

0−

  1. The sequence of the 3814-bp iNOS human promoter (H3.8), or the 1800-bp 5′ fragment (H1.8) analogous to the 1588-bp murine iNOS promoter previously reported by Xie et al. (16) was analyzed using a NALIGN program with the PRE consensus sequences listed. The PREs identified bind proteins induced in response to the activating factors LPS, IFNγ, IL-1β or TNFα used in the experiments reported here. The mouse sequence is presented for a more direct comparison of the number of resident PREs.
  2. a Base match present in sequence compared with the PRE consensus sequence. Where less than a perfect match is tabulated, the mismatch was also reported to be active as a PRE.
  3. b Number of PREs identified in the entire human 3.8-kb iNOS promoter region in either the positive (+) or negative orientation (−).
  4. c Number of PREs identified in the entire human 1.8-kb iNOS promoter region in either the positive (+) or negative orientation (−).
  5. d Number of PREs identified in the mouse 1.5-kb iNOS promoter region in either the positive (+) or negative orientation (−) derived from a previously published analysis.
  6. e Consensus sequence of PRE.