Element
|
Consensus Sequence
|
Ref.
|
n/n
a
|
Human 3.8-kb Clone
b
|
Human 1.8-kb Clone
c
|
Mouse 1.5-kb Clone
d
|
---|
γ IRE | CTKKANNYe |
24
| 8/8 | 11+ | 8− | 9+ | 1− | 4+ | 0− |
| | | 7/8 | 141+ | 140− | 50+ | 52− | 5+ | 1− |
AP-1 | TGACTCA |
25
| 7/7 | 0+ | 0− | | | | |
| | | 6/7 | 5+ | 4− | 3+ | 1− | 1+ | 1− |
NF-IL6 | AGTTANGNAA |
26
| 10/10 | 1+ | 0− | 1+ | 0− | | |
| | | 9/10 | 4+ | 2− | 3+ | 1− | | |
NF-κB | GGGRNNYYCC |
27
| 10/10 | 1+ | 0− | 1+ | 0− | 2+ | 0− |
TNF-RE | TGAGCTCA |
28
| 8/8 | 0+ | 0− | | | | |
| | | 7/8 | 5+ | n/a | 3+ | n/a | 2+ | n/a |
X-box | CCYAGMRACNG |
24
| 8/11 | 0+ | 0− | | | 1+ | 0− |
GAS | TTACTCTAAA |
24
| 8/10 | 0+ | 0− | | | 1+ | 0− |
IRF-E | SYAAAGYSAAA(A) G |
29
| 11/13 | 1+ | 0− | 0+ | 0− | 0+ | 0− |
| | | 10/13 | 0+ | 2− | 0+ | 2− | | |
ISRE | YAGTTTC (A/T) YTTTCC |
30
| 11/14 | 2+ | 2− | 1+ | 2− | 2+ | 0− |
- The sequence of the 3814-bp iNOS human promoter (H3.8), or the 1800-bp 5′ fragment (H1.8) analogous to the 1588-bp murine iNOS promoter previously reported by Xie et al. (16) was analyzed using a NALIGN program with the PRE consensus sequences listed. The PREs identified bind proteins induced in response to the activating factors LPS, IFNγ, IL-1β or TNFα used in the experiments reported here. The mouse sequence is presented for a more direct comparison of the number of resident PREs.
- a Base match present in sequence compared with the PRE consensus sequence. Where less than a perfect match is tabulated, the mismatch was also reported to be active as a PRE.
- b Number of PREs identified in the entire human 3.8-kb iNOS promoter region in either the positive (+) or negative orientation (−).
- c Number of PREs identified in the entire human 1.8-kb iNOS promoter region in either the positive (+) or negative orientation (−).
- d Number of PREs identified in the mouse 1.5-kb iNOS promoter region in either the positive (+) or negative orientation (−) derived from a previously published analysis.
- e Consensus sequence of PRE.