Fig. 2

Inhibition of trophozoite extract-derived heme polymerization activity by the heme analog ZnPP

Parasites were cultured at increasing ZnPP concentrations. Data are expressed as incorporation of radioactive heme into an insoluble polymer (hemozoin). Halfmaximal inhibitory effect (IC₅₀) of ZnPP is observed at a concentration of ≈ 500 µM. The line represents the nonlinear least squares fit to the data. Each data point represents mean ± standard errors of two data points from a representative experiment.