Correction of TNF and LTα production in TNF-LT
(-/-) deficient mice upon bone marrow transplantation from wild-type mice
(A) TNF plasma levels 1 hr after LPS injection. (B) In vitro TNF production by bone marrow-derived macrophages stimulated with LPS (3 hr, 10 ng/ml) in the presence of interferon γ (100 U). (C) TNF and LTα mRNA transcription in splenic T lymphocytes after in vitro concanavalin-A stimulation (5 µg/ml at 24 hr). Bone marrow cells (2 × 106) from wild-type mice (+/+) or from TNF-LTα knock-out (-/-) donor mice were transplanted into lethally irradiated wild-type (+/+) or TNF-LTα knock-out recipient mice (-/-). Analysis of the in vivo TNF production in response to LPS (10 µg i.p.) was performed 47 days post-transplantation, at a time point when the hematological parameters were stabilized (A). In Panels B and C, bone marrow and spleen cells were harvested on Day 84 post-transplantation for in vitro expansion and stimulation. Results are from one representative experiment and are expressed as mean ± SEM (n = 3–4).