Fig. 2
From: Macrophage Growth Factors Introduced into the Kidney Initiate Renal Injury
Subcapsular implantation of CSF-1-TEC and GM-CSF-TEC into MRL strain induces renal injury in mice with the lpr mutation
Implantation of CSF-1-TEC under the renal capsule caused a wedge-shaped intrarenal infiltrative lesion (A, arrows), and GM-CSF-TEC induced severe lesion extending through the cortex into the medulla (B, arrows) in MRL-lpr recipients. By comparison, neither CSF-1 (C) nor GM-CSF (D) induced any intrarenal lesion and only induced a modest accumulation of cells in the subcapsular sites in MRL-++ mice. Similarly, uninfected TEC implanted into MRL-lpr recipients (E) did not cause intrarenal lesion. β-gal-CSF-1-TEC implanted into MRL-lpr mice stained for the presence of β-gal are confined to the renal subcapsule 3 days post-Ix (F, arrows), and 14 days post-Ix (G, arrows). sc, subcapsular space. (Hematoxylin and eosin; magnification: 200×.)