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Fig. 1 | Molecular Medicine

Fig. 1

From: Immunophilin Regulation of Neurotransmitter Release

Fig. 1

FK506 inhibits release of acetylcholine (ACh) and dopamine from PC12 cells

PC12 cells were cultured and [3H]-neurotransmitters were released as described in Materials and Methods. Various concentrations of FK506 or FK506 + 1 mM rapamycin were added to the cells 5 min prior to addition of NaCl or KCl to initiate transmitter release. Release of [3H]-ACh (upper panel) and dopamine (lower panel) was quantified as described in Materials and Methods. The amount of transmitter released by addition of NaCl (basal release, diagonally lined bars) in absence of drug treatment was designated 100% of control. Neurotransmitter released by addition of KCl is indicated by white bars. *Statistically significant differences between drug treatment and controls (p < 0.05 using paired and unpaired Student’s t tests).

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