Skip to main content
Fig. 2 | Molecular Medicine

Fig. 2

From: Eotaxin Triggers Eosmophil-Selective Chemotaxis and Calcium Flux via a Distinct Receptor and Induces Pulmonary Eosinophilia in the Presence of Interleukin 5 in Mice

Fig. 2

Chemotaxis of murine eosinophils to recombinant eotaxin before or after Xa cleavage

(His)6-eotaxin purified from E. coli by Nickel and reverse phase HPLC chromatography was resuspended in water at 30 µg/ml. The chemotactic response of this protein was then tested before (Uncut) and after factor Xa digestion (Cut) without further purification. The chemotactic response to 100 ng/ml of MIP-1α and buffer alone are also indicated. The results are expressed as the mean ± SEM for replicate samples.

Back to article page