Chemotaxis of murine eosinophils to recombinant eotaxin before or after Xa cleavage
(His)6-eotaxin purified from E. coli by Nickel and reverse phase HPLC chromatography was resuspended in water at ∼30 µg/ml. The chemotactic response of this protein was then tested before (Uncut) and after factor Xa digestion (Cut) without further purification. The chemotactic response to 100 ng/ml of MIP-1α and buffer alone are also indicated. The results are expressed as the mean ± SEM for replicate samples.