Fig. 1

Thrombin receptor and PAR2 genomic structure

(A) Exon-intron organization. The exon-intron structures of the human and murine thrombin receptor genes are shown and compared to those reported for the murine and human PAR2 genes (6,8). Boxes represent exons with coding sequences filled and untranslated sequences open. Partial restriction maps for the human and mouse thrombin receptors are shown. Abbreviations for the restriction endonuclease sites are as follows: EcoRI (r), EcoRV (v), HindIII (h), KpnI (k), NotI (n), PstI (p). SacI (s), XhoI (x). (B) Thrombin receptor and PAR2 splice sites. The nucleic acid and deduced amino acid sequences at the splice sites for the mouse and human thrombin receptor genes are compared with those reported for mouse and human PAR2 (6,8). The consensus splice donor and acceptor sequences are underlined. Exon and cDNA sequences are shown in uppercase, intronic sequence in lowercase. Possible signal peptidase cleavage sites for the three receptors are indicated by arrowheads (28,29). The splice sites in these genes are located at similar positions in their receptor’s coding regions, in essence separating the putative signal peptides from the mature receptor proteins.