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Fig. 3 | Molecular Medicine

Fig. 3

From: Conserved Structure and Adjacent Location of the Thrombin Receptor and Protease-Activated Receptor 2 Genes Define a Protease-Activated Receptor Gene Cluster

Fig. 3

Human thrombin receptor and PAR2 genes are found on the same YAC clones

Four YACs containing both the thrombin receptor and PAR2 were identified by screening a human YAC library for both receptors by PCR. YAC or P1 DNA was digested extensively with the restriction enzymes EcoRI, Pst I and HindIII, as indicated. Southern analysis was performed at high stringency on 10 µg of total yeast DNA from yeast bearing the indicated YAC. Southern analysis was also performed using 100 ng of DNA from a P1 bacteriophage clone containing the only human thrombin receptor gene (control, upper panel) and on a separate clone containing only the human PAR2 gene (control, lower panel). The source of probe (either thrombin receptor or PAR2 exon 2) is shown on the left and size markers are on the right. The thrombin receptor P1 DNA showed no hybridization to PAR2 probe and the PAR2 P1 DNA showed no hybridization to thrombin receptor probe at high stringency (data not shown).

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